P761H - SARS-CoV-2 Nucleic Acid Direct Detection Kit
- Tianlong
- China
- 1-2 weeks
- 2000 units/M
The TianLong SARS-CoV-2 Nucleic Acid Detection Kit is intended for the qualitative detection of SARS-CoV-2 nucleic acid by Direct Real-time reverse transcription Polymerase Chain Reaction (Direct Real-time RT-PCR) method.
SARS-CoV-2 Nucleic Acid Detection Kit (Direct Real-time RT-PCR Method)
Intended Use
The TianLong SARS-CoV-2 Nucleic Acid Detection Kit is intended for the qualitative detection of SARS-CoV-2 nucleic acid by Direct Real-time reverse transcription Polymerase Chain Reaction (Direct Real-time RT-PCR) method.
The test is designed to detect RNA from SARS-CoV-2 in specimens directly (without nucleic acid extraction steps) such as nasopharyngeal or oropharyngeal swabs collected from individual personnel based on clinical and/or epidemiological criteria.
Product Advantages:
Direct amplification without RNA extraction ;
Rapid Testing and report in 45 minutes;
Product Parameters:
Product Name | SARS-CoV-2 Nucleic Acid Detection Kit (Direct Real-time RT-PCR Method) |
Cat.No | P761H |
Specification | 50T/Kit |
Specimen | Nasopharyngeal or oropharyngeal swabs |
Sensitivity | 1000 copies/ mL |
Target Gene | RdRP, N |
Storage & Validity | -25℃~-15℃ for 12 months |
Applicable Equipment | Instruments with FAM, VIC (HEX), Cy5 fluorescence channels such as Tianlong Gentier Real-time PCR Systems |
Accessories:
Product Name | Sample Collection Set |
Order Code | T319H |
Specification | 10T/ Kit |
Specimen | Nasopharyngeal swab or oropharyngeal swab |
Storage & Validity | Storage under room temperature; Validity for 12 months |
Usage | Storage and transportation of human specimen |
Note: Tianlong SARS-CoV-2 Nucleic Acid Detection Kit (Direct Real-time RT-PCR Method) (P761H) should be used together with Tianlong Sample Collection Set (T319H).
Principles of the Assay
The assay is designed to target the well-conserved regions of RNA-dependent RNA polymerase (RdRp) and N protein genes, for the most consistent assay performance over the course of epidemic evolvement in spite of the mutation prone nature of RNA viruses.
Specific primer and TaqMan probe sets are designed, with good tolerance for known virus strains and potentially evolving mutations of the SARS-CoV-2 virus. Nucleic acids are extracted from designated samples with adequate methods and materials. After reverse transcription, the presented target sequences of RdRp and N will be amplified by respective specific primers and detected by TaqMan probes. During the extension phase of each PCR cycle, the Taq polymerase cleave reporter dye off the 5' end of the probe, separate dye from the quencher, producing fluorescent signals. The increasing fluorescence signals are detected over the PCR course and data analyzed.
1.1 Sample throughout difference: Gentier96 can process maximum 96 samples per run, Gentier48 can process maximum 48 samples per run; 1.2 Fluorescence channel difference: Gentier96 E/R has 6/4 fluorescence channels separately, Gentier48 E/R has 4/2 fluorescence channels; 1.3 Light source location difference : The light source of Gentier96 is on the top, which requir...more
